South African praying mantis feeding on a wasp, photographed with the Canon EOS R5 Mark II
South African praying mantis|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM |1/100 sec · f/13 · ISO 160

South African praying mantis|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM |1/100 sec · f/13 · ISO 160

The limits are now set by our imagination, not the equipment.

In August 2024, Canon released the EOS R5 Mark II. The original R5 was already one of the best cameras on the market for wildlife photography at the time, but the Mark II has added features that have made a real difference to the macro and wildlife photography I do.

I specialise in macro photography of New Zealand's endemic flora and fauna, and I rarely get to photograph them under perfect conditions. Invertebrates move, plants sway in the wind, the light changes, and some subjects give you only split seconds to make the photograph. With the R5 Mark II, those conditions are easier to work around. Photographs I once would not have attempted are now possible, and the camera is less often the limiting factor.

Male Puwere pureora photographed with the Canon EOS R5 Mark II
Male Puwere pureora|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM|1/125 sec · f/5.6 · ISO 250 · 12-image focus stack

Depth exactly where I want it

In-camera focus bracketing lets me build depth through a subject while using apertures that retain more detail, and guide the viewer's eye to the details that matter.

The trade-off between depth and sharpness

Every macro photographer faces the same trade-off. Stopping down gives more depth of field, but less light reaches the sensor and diffraction begins to soften the image.

For the shorter stacks I often use in the field, f/11 usually gives me the best balance between depth of field and sharpness. Some photographers stop down to f/16, but in my experience the extra depth is limited and not worth the loss of light and sharpness. I do not see a real improvement in depth until around f/25, and by then there is a noticeable drop in sharpness.

Photographers using other camera systems can achieve excellent results at f/25. My good friend Danilo Hegg of Southern Alps Photography is one of them. Every camera system and lens behaves differently.

Before in-camera focus bracketing

Before in-camera focus bracketing, I had to turn the focus ring by hand or move the camera between frames. It was slow and could leave uneven steps, with bands of missed focus.

I still use manual-focus lenses such as the Canon MP-E 65mm in the field. Now that the Mark II’s electronic shutter works with flash, I can capture frames much faster than before. For precise movement at magnifications higher than 1:1, such as 2:1 or 3:1, or when working with manual-focus lenses, an automated rail such as the StackShot is still the better option. It is a heavy piece of kit and needs a very stable platform. With an autofocus lens, the R5 Mark II can handle the focus movement in camera with one press of the shutter.

Cognisys StackShot macro rail package StackShot macro rail package, Cognisys →

There is no one rule for every subject. These are the settings that work for me. The right combination is trial and error, depending on subject size and depth of field, so it is worth finding what works for you by testing in a controlled environment at home.

Key tipFocus bracketing switches off when the camera is turned off, so it is worth checking it is still enabled before an important photograph. Canon's focus bracketing guide →

SettingRangeWhat it doesHow I use it
Number of shots2–999How many frames the camera captures in the sequence.For most photographs, I use up to 15 frames with flash, occasionally up to 25 frames. In natural light, I use around 25 frames, depending on the depth of the subject.
Focus increment1–10How far focus moves between frames. Lower values give smaller steps with more overlap.I've found 6 or 7 works well for handheld and tripod flash work, depending on the subject's size, and 5 or 6 for natural light on a tripod.
Exposure smoothingOn / OffCorrects small brightness changes between frames as the effective aperture shifts with focus position.On with the RF 100mm. Canon's original R5 manual advises turning it off with the EF 100mm f/2.8L Macro, EF 180mm f/3.5L Macro and EF-S 60mm Macro, as brightness can change.
Depth compositeOn / OffMerges the frames into a finished JPEG or HEIF in camera, while keeping the source images.Off depending on the situation. I blend my photographs in applications such as Zerene Stacker or Helicon Focus.
Flash interval0 or longerThe time between frames when using flash.0 with the EL-1. Each frame fires as soon as the flash is ready. More in the next section →

Choose the depth the photograph needs

Focus bracketing is not about making every millimetre of the subject sharp. It is about putting the depth where it tells the story.

High-magnification focus stack photographed at four times life size
New Zealand Calloused Beach Pillbug (Tylios neozelanicus)|4× life size|Canon EOS R5 Mark II + MP-E 65mm f/2.8 1–5x Macro Photo|1/160 sec · f/5.6 · ISO 250 · 16-image focus stack

Stacking tips

Start at the nearest detail

Start at the closest point you want sharp. Focus only moves away from you, so anything in front of it won't be sharp.

Keep it short

I only use enough frames to get what I need in focus. Too many frames can cause alignment issues, halos and other stacking artefacts.

Further reading

The Helicon Soft forum and Photomacrography.net have an amazing amount of information and resources on focus stacking.

Focus bracketing with flash

For me, one of the most useful changes is being able to use flash during focus bracketing.

Green and golden bell frog photographed with a seven-image flash focus stack on the Canon EOS R5 Mark II
Green and golden bell frog (Ranoidea aurea)Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM + Speedlite EL‑1|1/125 sec · f/5.6 · ISO 250 · 7-image focus stack

A seven-frame stack in under a second

I captured this focus stack with flash in under a second, just moments before the frog jumped away. It was one of those brief opportunities where everything just worked in that split second: focus, bracketing and flash. The R5 Mark II was fast enough with flash that I could capture the full series of images for the stack before the frog moved.

Flash, now with the electronic shutter.

The original R5 already had very capable in-camera focus bracketing, but it could not fire flash during the sequence. The Mark II changes that.

With a compatible Speedlite, each frame can be lit consistently while helping to freeze subject movement. The camera can capture these sequences quickly enough to be useful with live subjects, letting me attempt flash-lit stacks in the field that would previously have been difficult.

Why the flash has to keep up

If the flash can't keep up, you lose much of the speed that makes this so useful when photographing live subjects.

Flash usedCanon Speedlite EL-1 →
Courtaraneus orientalis orb-web spider photographed with focus bracketing and flash on the Canon EOS R5 Mark II
Courtaraneus orientalis|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM + Speedlite EL‑1|1/125 sec · f/8 · ISO 100 · 9-image focus stack
0flash interval setting I use
0.1–0.9 secEL-1 recycling time (Canon)
≈170continuous full-power flashes (Canon)

I nearly always use flash through a diffuser for macro photography. I use a couple of Beetle Diffuser models, and their diffusion material gives me soft, even light while still allowing enough output for fast focus-bracketing sequences.

I still build and use my own custom diffusers depending on the subject and working distance, but I now use the same diffusion material used in the Beetle Diffusers. I liked the material enough that I bought it directly from Varun to use in my own designs.

Some opportunities you only get once

Leaf-vein slug photographed in New Zealand
Leaf-vein slug (Athoracophorus sp.) — mating|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM |1/125 sec · f/11 · ISO 250 · 3-image focus stack
Freshly moulted chorus cicada, Amphipsalta zelandica, photographed with focus bracketing on the Canon EOS R5 Mark II
Chorus cicada (Amphipsalta zelandica) — freshly moulted|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM |1/100 sec · f/7.1 · ISO 250 · 13-image focus stack
South African praying mantis feeding on prey
South African praying mantis|Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM|1/100 sec · f/13 · ISO 160

Flash control when the subject moves

Close flash gives me the working aperture and short duration needed for a moving subject, while the diffuser keeps the light controlled and natural-looking.

Finding the balance

There is no single setting that works for every subject. I normally adjust the number of frames, aperture and flash power depending on how much time I have and how likely the subject is to move.

Number of framesFewer frames = less time to capture
ApertureUsually around f/5.6
Shutter speedUsually 1/125 sec for reliable flash sync
Flash powerUsually –2 E-TTL or 1/32 manual
ISOUsually ISO 200, adjusted as needed

Keeping it quick

I normally set the flash interval to 0, so the camera takes the next frame as soon as the EL‑1 is ready. Keeping the flash power low also helps keep the process quick.

E-TTL or manual?

Manual for consistent output; E-TTL when distance or composition is changing. More detail below.

A few practical considerations

Focus-bracketed work can use a lot of battery power and create plenty of files, so I always carry spare camera and flash batteries, along with plenty of card space.

Flash control in practice

Manual flash

Manual is my usual choice when I want consistent output from one frame to the next. With the Beetle Pro diffuser, I often begin at 1/32 power. It means a dark background does not persuade the flash to overpower the subject, and I can make small changes deliberately.

E-TTL flash

I use E-TTL when the subject distance or composition is changing quickly. I often begin at –2 flash exposure compensation, then adjust after looking at the frame. The EL-1 Select dial makes those changes quick in the field.

Behaviour that lasts a split second

The moment a predator grabs its prey happens in the blink of an eye.

Cormocephalus westwoodi huttoni centipede with prey, photographed with the Canon EOS R5 Mark II
Cormocephalus westwoodi huttoni with preyCanon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM|1/125 sec · f/5.6 · ISO 250 · focus stack
30 fpselectronic shutter
12 fpsmechanical shutter
≈0.5 secpre-capture before the full press

More chances to capture it

A feeding interaction can change from one frame to the next: the prey moves, a bird's wings shift, or the behaviour changes in a split second. I can't ask the subject to repeat the moment. Shooting at up to 30 fps with the electronic shutter gives me far more frames to choose from when selecting the one that best shows the predator and its prey. I still have to anticipate the action and keep the subject in frame, but the extra speed gives me a much better chance of capturing the exact moment I'm after.

Recording the moment before I react

Moments like this can happen faster than I can react. A bird can pick up, toss and swallow food in a fraction of a second, and by the time I fully press the shutter the moment I wanted may already have happened. Pre-continuous shooting helps by capturing the frames immediately before I fully press the shutter, giving me a much better chance of catching the exact point in the action I was looking for.

Bird feeding, captured with pre-continuous shooting on the Canon EOS R5 Mark II
Rose-crowned Fruit Dove (Ptilinopus regina)|Canon EOS R5 Mark II + RF 100–500mm F4.5–7.1 L IS USM|1/640 sec · f/9 · ISO 6400
≈0.5 secrecorded before the full press
15frames kept from before the press
30 fpselectronic shutter
RAWas well as JPEG and HEIF

Pre-continuous shooting starts buffering frames while the shutter button is half-pressed. When I press it fully, the camera keeps about half a second of frames from before that moment, then carries on shooting at 30 fps.

Pre-Continuous Shooting: Pre-continuous shooting uses the electronic shutter, and flash can't be used with it. For flash-lit bursts, I switch back to normal continuous shooting.

Autofocus that locks on when it matters

The autofocus finds and holds the subject quickly, whether it is a bird in flight or a small invertebrate on a leaf. The original R5 already had very capable autofocus; the Mark II improves it further.

Male wolf spider, Tasmanicosa semicincta, photographed close-up with the Canon EOS R5 Mark II
Male wolf spider (Tasmanicosa semicincta) Canon EOS R5 Mark II + RF 100mm F2.8 L Macro IS USM + Raynox DCR-250 | 1/125 sec · f/11 · ISO 250 · 15-image focus stack

A tiny target

With this wolf spider, the eye is where I want the viewer's attention. At macro distances, it is a tiny target with very little depth of field. The autofocus gives me a stronger starting point, even when the animal is moving or the background is busy. It does not replace careful technique, but finding the eye sooner gives me more time to make a focus stack.

Rotorua harvestman, Sorensenella rotara, photographed in New Zealand

Rotorua harvestman (Sorensenella rotara)
Canon EOS R5 Mark II + Speedlite EL-1 + DIY diffuser | 1/125 sec · f/5.8 · ISO 200 · 8-image focus stack

Eyes found when the subject blends in

This female Trite sp. was barely 6 mm long, but the autofocus found and held her eyes.

6 mm female Trite jumping spider photographed with the Canon EOS R5 Mark II 6 mm female Trite jumping spider on a leaf of almost the same colour
Trite sp., female | Canon EOS R5 Mark II + LENS | SETTINGS

The spider and the leaf it was sitting on were almost the same colour, which would usually make autofocus more difficult.

Being able to find the eye quickly gives me a better starting point when the subject is small, moving, or partly hidden.

Detail that helps distinguish similar species

Resolution is not only about large prints. It lets me look closely at the small features that can matter when identifying and documenting an animal.

Triregia fairburni on moss

Triregia fairburni | A detailed photograph creates a useful record.

Eye arrangement

Often one of the quickest ways to separate similar-looking species.

Spines and markings

Small structural details can be difficult to assess while the animal is moving.

Shape and structure

A high-resolution frame gives me room to inspect and crop carefully later.

A viewfinder that makes the scene easier to read

The electronic viewfinder has the same 5.76-million-dot resolution as the original R5, but it is approximately twice as bright. In the field, that makes a real difference when I am working in low light or trying to judge fine detail on a small subject.


At up to 120 fps, the view stays more fluid when I am following a moving bird or invertebrate. It does not sound like a major change on a specification sheet, but it makes the camera feel more immediate to use.

Canon R5 Mark II electronic viewfinder

Canon R5 Mark II electronic viewfinder | The brighter viewfinder makes fine detail easier to read.

Natural-light macro

The R5 Mark II is not only about flash. These fungi photographs were made in natural light, where the small changes in colour, texture and depth are just as important.

Yellow fungiGreen fungiRed fungiBlue pinkgill fungus

What this camera lets me attempt

For the way I photograph, the R5 Mark II is not simply a specification upgrade. It lets me work with moving subjects, build useful depth in the field and keep the point of focus exactly where I want it. It has made more of the photographs I care about possible.

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